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(joint with Nanotech 2006)
High processivity DNA polymerase
| Organization: | Rice Unviersity, TX, US |
| I.P. Brief: | The invention relates to a high-processivity DNA polymerase obtained by fusing a bacteriophage DNA polymerase with a single stranded DNA binging protein (ssDNAbp). The fusion of a ssDNAbp significantly increases the enzyme’s processivity without negatively affecting fidelity. |
| Summary of I.P.: | PCR technology is a ubiquitous tool in every life science laboratory, and is widely used for both general research purposes and specific applications such as forensic DNA testing, and microbial and pathogen detection. As PCR applications become more common in diagnostics applications, the need for high-performance enzymes increases. In particular, improved processivity leads to higher PCR yields and faster overall run times, hence saving time and improving template integrity by minimizing exposure to extreme cycling temperatures. The invention relates to a high-processivity DNA polymerase obtained by fusing a bacteriophage DNA polymerase with a single stranded DNA binging protein (ssDNAbp). The fusion of a ssDNAbp significantly increases the enzyme’s processivity without negatively affecting fidelity. Interestingly, a ssDNAbp can be covalently linked to a fusion double stranded DNA binding protein – DNA polymerase, as utilized in several commercial products for PCR. Benefits: Enhanced processivity. Improved template integrity. Faster PCR of longer DNA fragments. |
| Patent: | |
| Keywords: | PCR, biotech, enzymes, molecular biology |
| Primary Industry: | Pharma & Biotech |
| Specific Market: | PCR, molecular biology |
| Market Size: | The enzymes used in PCR have generated 250 million dollars in profit for Roche and over 2 billion dollars overall, since 1991. The applications range from biological research, to laboratory medicine, forensic medicine and diagnostics. |
| State of the Art: | na |
| Figures of Merit: | na |
| Tech. Obstacles: | None |
| Market Obstacles: | None |
| Publications: | na |
| Research Team: | 2 |
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