Venue | Press Room | Subscribe | Site Map
TechConnect Summit 2006
Home | About the Summit | Program | Advisory Boards | About TechConnect

Producing Sponsor

TechConnect

Platinum Sponsor

Welsh & Katz, Ltd.

Gold Sponsor

ISTC International Science & Technology Center

Ventures Enabling Sponsor

Atomic Venture Partners

Press Sponsor

Business Wire

Partnering Sponsor

NSTI

Supporting Organizations

Boston University
Midwest Research University Network

Media Sponsors

(joint with Nanotech 2006) Nature
Science
R&D Magazine

Summit News

Event Contact

Jennifer Rocha
955 Massachusetts Ave. #313
Cambridge, MA 02139
Phone: (774) 249-8514

High processivity DNA polymerase

Organization:Rice Unviersity, TX, US
I.P. Brief:The invention relates to a high-processivity DNA polymerase obtained by fusing a bacteriophage DNA polymerase with a single stranded DNA binging protein (ssDNAbp). The fusion of a ssDNAbp significantly increases the enzyme’s processivity without negatively affecting fidelity.
Summary of I.P.:PCR technology is a ubiquitous tool in every life science laboratory, and is widely used for both general research purposes and specific applications such as forensic DNA testing, and microbial and pathogen detection. As PCR applications become more common in diagnostics applications, the need for high-performance enzymes increases. In particular, improved processivity leads to higher PCR yields and faster overall run times, hence saving time and improving template integrity by minimizing exposure to extreme cycling temperatures. The invention relates to a high-processivity DNA polymerase obtained by fusing a bacteriophage DNA polymerase with a single stranded DNA binging protein (ssDNAbp). The fusion of a ssDNAbp significantly increases the enzyme’s processivity without negatively affecting fidelity. Interestingly, a ssDNAbp can be covalently linked to a fusion double stranded DNA binding protein – DNA polymerase, as utilized in several commercial products for PCR. Benefits: Enhanced processivity. Improved template integrity. Faster PCR of longer DNA fragments.
Patent:
Keywords:PCR, biotech, enzymes, molecular biology
Primary Industry:Pharma & Biotech
Specific Market:PCR, molecular biology
Market Size:The enzymes used in PCR have generated 250 million dollars in profit for Roche and over 2 billion dollars overall, since 1991. The applications range from biological research, to laboratory medicine, forensic medicine and diagnostics.
State of the Art:na
Figures of Merit:na
Tech.  Obstacles:None
Market Obstacles:None
Publications:na
Research Team:2

 

Home | About the Summit | Program | Advisory Boards | About TechConnect
Terms of use | Privacy policy | Contact | TechConnect Home
© 2005 TechConnect. All rights reserved.