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Detection of S-nitrosylated and Glutathionylated Proteins

Organization:University of Vermont, VT, US
I.P. Brief:Methods and materials for functional detection of S-nitrosylated and glutathionylated proteins in intact tissues and cells.
Summary of I.P.:There is no direct method for detection of these modifications in intact cells and tissues and current antibodies are unspecific. Fuctional detection is critical in the investigation of of the role these modifications play in normal and disease affected cells and may be diagnostic for certain diseases. Hospitals and research centers can use this invention to determine functional NO in cells or tissues from patients or animals. This may serve as a diagnostic tool in diseases, and can also evaluate the effectiveness of NO-delivery strategies.
Patent:11/104,387, PCT/US2005/012259
Keywords:Nitric oxide, nitrosylation, glutathionylation, diagnostics
Primary Industry:Pharma & Biotech
Specific Market:The research with potential for cancer and heart diagnostics
Market Size:500K to 1M
State of the Art:The current methods use an antibody directed against S-nitrosocysteine which has questionable specificity, as the NO-cysteine bond (known as S-nitrosylation or S-nitrosation) is highly labile. There is no current method for intact tissues and cells.
Figures of Merit:It provides a specific and direct method of detection of these bonds and can do it in intact cells and tissues.
Tech.  Obstacles:None for slide mounted cells and tissues. Developing fluid based assay.
Market Obstacles:Packaging and testing for the research market. Clinical studies for diagnostic support. Development of fluid based assay. High-throughput assay development.
Publications:Reynaert NL, Ckless K, Guala AS, Wouters EF, van der Vliet A, Janssen-Heininger YM. \"In situ detection of S-glutathionylated proteins following glutaredoxin-1 catalyzed cysteine derivatization.\" Biochim Biophys Acta. 2006 Feb 20 Ckless K, Reynaert NL, Taatjes DJ, Lounsbury KM, van der Vliet A, Janssen-Heininger Y. \"In situ detection and visualization of S-nitrosylated proteins following chemical derivatization: identification of Ran GTPase as a target for S-nitrosylation.\" Nitric Oxide. 2004 Nov;11(3):216-2
Research Team:The Laboratory of Yvonne Janssen-Heininger at the University of Vermont http://www.med.uvm.edu/pathology/WebBio.asp?SiteAreaID=617

 

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