|Organization:||University of Vermont, VT, US|
|I.P. Brief:||A slide based totally internal reflected fluorescence microscopy (TIRFM) platform that uses a fiber optic light guide to launch laser light into a microscope slide to produce an evanescent energy gradient suitable for TIRFM. The platform can be used on nearly any microscope with little or no modifications needed.|
|Summary of I.P.:||TIRFM has a fast growing user group in the scientific and research and development communities anxious to use its high spatial resolution imaging of objects and reduction of background fluorescence. The ability to grow the current user group for TIRFM is limited by the high costs of the high numerical aperture objective lenses and highly processed set of associated optics currently needed to do TIRFM. This simple, inexpensive, disposable microscope slide approach provides a low cost alternative, as well as the possibility of a disposable component for current TIRFM flow chambers eliminating difficult and time consuming cleaning processes.|
|Keywords:||TIRFM, fiber optic, evanescent wave, laser, polarization|
|Primary Industry:||Health & Medical Devices|
|Specific Market:||Research and development in multiple fields|
|State of the Art:||High numerical aperture objective lenses and highly processed sets of associated optics are used to create TIRFM.|
|Figures of Merit:||This simple, inexpensive, disposable microscope slide approach provides a low cost alternative, as well as the possibility of a disposable component for current TIRFM flow chambers eliminating difficult and time consuming cleaning processes.|
|Tech. Obstacles:||Testing in a variety of uses. |
|Market Obstacles:||Test prototype in a variety of use situations.
Development of products for each.
Manufacturing processes for each.
|Research Team:||Guy Kennedy and David Warshaw at the University of Vermont http://www.uvm.edu/cmb/faculty_details.php?people_id=119|