S. Dixit, M.D. Davis, S.R. Singh, V.A. Dennis
Alabama State University, United States
pp. 302 - 305
Keywords: polyvinyl alcohol (PVA), poly (lactic acid)-b-Poly (ethylene glycol)
Polyvinyl alcohol is the most commonly used emulsifier in the formulation of NPs. Provides non-aggregating, emulsifying and adhesive properties to NPs and an interconnected network with the polymer at the interface which ultimately can affect their homogeneity, release profile and size. The objective of this study was to determine the concentration-dependent effect of PVA on the size, morphology and release profiles of the modeled protein, bovine serum albumin encapsulated within PLA-PEG. NPs were formulated by the Water/Oil/Water double emulsion method using concentrations of PVA ranging from 0.5-3%. Scanning electron microscopy analyses revealed that use of 0.5, 1.0 and 2% PVA resulted in smaller NPs (105 - 228 nm). In contrast, 3% PVA resulted in larger NPs (246 - 332 nm). The encapsulation efficiency of NPs with 0.5, 2 and 3 % PVA was 10-30%, while that of 1% PVA was 40%. Selection of 1% PVA for formulation of NPs with 2X polymer (200 mg polymer with 4 mg BSA) and less BSA (2 mg BSA and 100 mg polymer) resulted in 55% and 64% EE, respectively. These interesting observations will permit the selection of sizes of PLA-PEG NPs formulations for maximal interaction with APCs, but more importantly for vaccine delivery.