S. Mozneb, J.C.K. Lai, S.W. Leung
Idaho State University, United States
pp. 155 - 158
Keywords: cyanide, biocomposite, performance, Au nano particles, biosensor, homeland security
Cyanide is an important industrial chemical but is also very toxic. Cyanide binds strongly with hemoglobin than that of oxygen which causes histoxic hypoxia, if cyanide is ingested into the bloodstream. This pairing reaction can thus be utilized for the development of a cyanide detection sensor. Our research group has been developing a sensor platform that can utilize different enzymatic couplings for the detections of metabolites and species that are of health and environmental concerns at extremely low concentrations. In this study, we report the detection of cyanide using our sensor platform with a biocomposite layer made up of polymers, nanogold sol-gel, and hemoglobin. The biocomposite is coated on the surface of anchoring materials such as Au, Pt, and glass carbon electrode. This ultra high sensitive biosensor can detect cyanide at concentration levels orders of magnitude lower than any reports found in the public domain. This report will reveal results in twofold: at high concentration levels of 1x10-6 M and low levels of 1x10-12 M, depending on the applications. We will also discuss factors that affect the sensitivity, interference, durability, and performance enhancement of this sensor.