Biotech, Biomaterials and Biomedical: TechConnect Briefs 2015Biotech, Biomaterials and Biomedical TechConnect Briefs 2015

Sensors, Diagnostics & Imaging Chapter 3

Comprehensive Analysis of High Performing Electrochemical Biosensors and Their Applications

D. Assan, J.C.K. Lai, S.W. Leung
Idaho State University, United States

pp. 145 - 147

Keywords: metabolites, diagnosis, nanoparticles, biosensor, environmental monitoring

We have mounted 5 different biocatalysts on a sensor platform to examine the performance of this electrochemical sensing system for the detection of different biomolecules/metabolites and environmental important molecules, with such we also compared how this sensing system fares with literature results of similar measurements. The biocatalysts are LDH (lactate dehydrogenase), GDH (glutamate dehydrogenase), human IgG (human immunoglobulin G), Hb (hemoglobin), and PSA (prostate-specific antigen). The sensor platform constitutes of a layer of biocomposite mounted on different electrodes made out of Au, Ag, Pt, and glass carbon; the biocomposite is fabricated with polymers and sol-gel Au nanoparticles with or without an extra layer of biomolecules. The targeting species for measurements include NH4+, NO3-, CN-, H2O2 and the biomolecules specific to the biomolecules/antigens coated on the surface of the biocomposites. In this report, we will provide a comprehensive analysis of this sensing system, including the unique identification potentials and sensitivities. This sensing system can be a valuable tool in biomedical diagnosis and environmental forensics