Biotech, Biomaterials and Biomedical: TechConnect Briefs 2015Biotech, Biomaterials and Biomedical TechConnect Briefs 2015

Biomaterials Chapter 5

Preparation and investigation of nano- carbonated hydroxyapatite/polymer composite spongy scaffolds for biomedical applications

S.A. Siddiqi, F. Manzoor, U. Azhar, A. Jamal, M. Tariq, M.S. Anwar, M. Saleem, A.A. Chaudhry, I.U. Rehman
COMSATS Institute of Information Technology, Pakistan

pp. 270 - 273

Keywords: hydroxyapatite, freeze drying, mesenchymal stem cell, spongy scaffolds, cell viability

Spongy scaffolds containing nano-Carbonated Hydroxyapatite (nCHA), with composition Ca10(PO4)6-y(CO3)y(OH)2,with y=2,4, were prepared by the lyophilization method. Different polymers, natural gelatin (G), chitosan (CS) and polyvinyl alcohol (PVA), alone or in combination, were used to make their composites with nCHA. All the samples were characterized by (XRD), (SEM), (FT-IR) techniques. Particle size and surface area were determined by BET surface area analysis. The nCHA containing scaffolds mediated cytotoxicity (MTT assay) and cell attachment studies (SEM) were carried out on rat bone marrow derived mesenchymal stem cell (MSC). SEM studies revealed varying microstructures for pore sizes and shapes pore interconnectivity. All the scaffolds prepared by freeze drying were found to be highly porous, porosities beyond 70%. Spongy scaffolds exhibited varying significantly pore morphology from one nCHA/polymer combination to the other one. Furthermore we investigated the biocompatibility of these scaffolds by performing cell viability and cell attachment tests. Our results indicated evident cell attachment on all the materials and also no significant cytotoxicity was observed in cells grown with these materials compared with control.The preparation of spongy scaffolds and their biocompatibility augment their potential use in tissue engineering and biomedical applications