Advanced Manufacturing, Electronics and Microsystems: TechConnect Briefs 2015Advanced Manufacturing, Electronics and Microsystems TechConnect Briefs 2015

Micro, Nano & Bio Fluidics Chapter 9

Negative Dielectrophoretic Force based High Efficiency and High Throughput Cell Sorting System Harnessing Paired Electrode Arrays and a Meso Size Channel

D. Lee, B. Hwang, B. Kim
Korea Aerospace University, Korea

pp. 339 - 342

Keywords: dielectrophoresis, negative dielectrophoretic force, cell sorting system, paired electrode array

Dielectrophoresis activated cell sorters (DACS), which exploit intrinsic dielectric properties of target cells, are highlighted as an alternative cell sorting method. Nonetheless, the DACS performing in micro channel have limitation with throughput while they accomplished high efficiency. In this paper, we present a high efficiency and throughput cell sorting system employing repeated electrode arrays and a meso size channel. The paired twin electrode arrays generate negative dielectrophoretic (n-DEP) force and deflect cells according to dielectric properties. The proposed DACS can achieve high efficiency even under strong hydrodynamic force caused by large flow rate for high throughput because target cells are continually deflected by n-DEP force while passing through each electrode pair. Consequently, the target cells, K562 cells, are effectively separated along electrode pair under voltage of 7 Vp-p with 100 kHz and flow rate of 30 μl/min and 50 μl/min in each outlet. The proposed sorting system showed high deflection ratio of 98.0±0.3% and high throughput over 38,000 cells/min. Additionally, we evaluated the proposed sorting system using mixture of K562 cells treated by Arsenic trioxide (As2O3). Conclusively, the sorting system achieved separation efficiency of 95%.