M. Kwon, Y. Park, S-Y Lee, Y.T. Kim, J.H. Lee
Luminescent MD, LLC, United States
pp. 152 - 154
Keywords: graphene sheet, chemiluminescence resonance energy transfer (CRET), aptamer, mercury
Using intra chemiluminescent resonance energy transfer (CRET) between DNA aptamer and fluorescein and physical property of graphene sheet, cost-effective and simple biosensor was developed for the rapid quantification of Hg2+ in a sample. The limit of detection (LOD = background + 3) for the biosensor with extremely low background, due to the removal of free DNA aptamer using graphene sheet, was compared with those of conventional analytical methods capable of quantifying Hg2+. The accuracy, precision, and recovery of highly sensitive biosensor were excellent within acceptable error range.