Advanced Materials: TechConnect Briefs 2015Advanced Materials TechConnect Briefs 2015

Nanoparticle Synthesis & Applications Chapter 5

Breast cancer diagnosis system using the combination of carcinoembryonic antigen aptamer, horseradish peroxidase-mimic hemin, and graphene sheet

S. Chong, K. Cho, H.J. Khang, S-Y. Lee, Y.T. Kim, J.H. Lee
Luminescent MD, LLC, United States

pp. 294 - 297

Keywords: graphene, pi-pi-stacking, aptamer, breast cancer, CEA

In order to early diagnose breast cancer with the rapid quantification of carcinoembryonic antigen (CEA) in human serum, highly sensitive DNA aptasensor with 1,1-oxalyldiimidazole chemiluminescence (ODI-CL) detection was developed using the combination of CEA aptamer, horseradish peroxidase (HRP)-mimic hemin, and graphene sheet in a polystyrene well. The accuracy, precision, and recovery of DNA aptasensor, capable of sensing CEA as low as 0.1 ng/ml, were good. Based on the research result, we confirmed that the novel DNA aptasensor can be applied as a new enzyme free analytical method for the early diagnosis of breast cancer.