Biotech, Biomaterials and Biomedical: TechConnect Briefs 2015Biotech, Biomaterials and Biomedical TechConnect Briefs 2015

Materials for Drug & Gene Delivery Chapter 1

PLGA and PLGA-PEG nanoparticles loaded with curcumin and their effect on aflatoxin B1 in vitro

A. Rivero-Gonzalez, R. Diaz-Torres, P. Ramirez-Noguera
Universidad Nacional Autonoma de Mexico, Mexico

pp. 56 - 59

Keywords: PLGA-PEG nanoparticles, curcumin, oxidative stress

The aim of this study was to evaluate the effect of nanoparticles prepared with polylactic-co-glycolic acid (PLGA) and PLGA with polyethylene glycol (PLGA-PEG) loaded with curcumin in human hepatocytes exposed to aflatoxin B1 in vitro. Some biomarkers associated to oxidative stress such as the enzymatic activity of catalase (CAT), glutathione (GSH) and lactate dehydrogenase (LDH) released into the medium in HepG2 cells exposed to concentrations of free curcumin and curcumin loaded nanoparticles were estimated. The results showed an increase in CAT activity and GSH concentration in cells exposed to nanoparticles with curcumin. PLGA-PEG nanoparticles showed more significant difference compared to control. Also decreased the amount of LDH. These results suggest that curcumin loaded nanoparticles can modulate cellular effects associated with oxidative stress in vitro.