O. Taratula, C. Schumann, T. Duong, K.L. Taylor, O. Taratula
Oregon State University, United States
pp. 106 - 109
Keywords: theranostic, NIR imaging, photothermal therapy, photodynamic therapy, image-guided surgery, naphthalocyanine
Currently, only optimal surgical resection of all disease sites can improve survival of ovarian cancer patients. Even with the best microsurgical techniques, however, palpation and visual inspection for malignant tissue identification can result in microscopic tumors being left behind that lead to cancer relapse. If these tumors could be detected, resected and treated more efficiently during surgery, survival rates would significantly increase. Therefore, we developed a single agent-based nanoplatform (NP) that can be used concurrently for two purposes: (1) tumor delineation with real-time near infrared (NIR) fluorescence signal and (2) intraoperative targeted treatment to further eliminate unresected cancer cells by non-toxic combinatorial phototherapy. The nanoplatform contains only three components: (1) naphthalocyanine (SiNc) as NIR imaging and phototherapeutic agent; (2) dendrimer as the SiNc carrier, and (3) polyethylene glycol as an enhancer of nanoplatform stability and biocompatibility. Efficiency of NP as an NIR-imaging agent was confirmed in vivo by recording the strong fluorescence signal in the tumor area. Anticancer efficacy of the combinatorial phototherapy was also demonstrated in nude mice bearing an ovarian cancer xenograft. The tumors treated with a single dose of SiNc-NP combined with 10 min of NIR light irradiation were completely eradicated from the mice.