Biotech, Biomaterials and Biomedical: TechConnect Briefs 2015Biotech, Biomaterials and Biomedical TechConnect Briefs 2015

Micro, Nano & Bio Fluidics Chapter 4

The application of 3D printing to study microfluidic architecture for ‘on chip’ mixing systems for SRCD and UV spectroscopy

M. Prince, P. Docker, C. Burton, R. Hussain, J. Kay, G. Silgardi
Diamond light source, United Kingdom

pp. 198 - 201

Keywords: UV spectroscopy 3D printing micro fluidics

Circular dichroism (CD) is the differential absorption of left- and right-handed circularly polarized light. It is a form of spectroscopy used to determine the optical isomerism and secondary structure of molecules, and to study a wide variety of chiral materials in solution, particularly biologically important molecules such as proteins, nucleic acids, carbohydrates, lipids and drugs. The benefit of carrying out such experiments using synchrotron radiation is that the light available is several orders of magnitude higher in intensity than that available using conventional CD instruments, thereby providing a much higher signal-to-noise ratio over a wide wavelength range (140-700 nm). This paper will detail the development of a technique for rapidly producing 3D printed microfluidic channels in transparent polymer flow cells that enables the rapid and low-cost evaluation and iteration of microfluidic channel geometries. Permitting the flow through novel microfluidic devices to be interrogated thoroughly and the design optimized prior to making the significant financial and most crucially time commitment to the UV compatible flow cell for presentation to the synchrotron.