J.T. Rashkow, Y. Talukdar, G. Lalwani, B. Sitharaman
Stony Brook University, United States
pp. 267 - 269
Keywords: inorganic nanoparticles, stem cells, cytotoxicity, differentiation, uptake, tissue engineering
We report the effects of one and two dimensional transition metal dichalcogenide nanoparticles, tungsten disulfide nanotubes (WSNTs) and molybdenum disulfide nanoplatelets (MSNPs), on fibroblastic cell (mouse fibroblasts (NIH-3T3) and human adipose derived stem cell (MSC)) viability and MSC differentiation potential. Cytotoxicity of MSNPs and WSNTs dispersed in distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)] (DSPE-PEG) was assessed by Presto Blue and lactate dehydrogenase (LDH) viability assays at concentrations ranging from 5 to 300 μg/ml for 6, 12 or 24 h. Viability assays showed no dose or time dependent increase in toxicity for NIH-3T3 cells treated with WSNTs or MSCs treated with MSNPs or WSNTs. Viability of NIH-3T3 cells treated with MSNPs at concentrations above 50 µg/ml was significantly lower compared to untreated cells. Analysis of MSCs differentiation to adipocytes and osteoblasts showed that MSC differentiation potential was not significantly affected after treatment with potentially safe low (10 µg/ml) and high (50 µg/ml) doses of MSNPs and WSNTs for 24 h. TEM analysis showed that both MSNPs and WSNTs are internalized into the cytoplasm by MSCs. The results lay the foundation to further explore the potential of these nanoparticles at potentially safe doses as multifunctional agents for biomedical applications.