Biotech, Biomaterials and Biomedical: TechConnect Briefs 2017Biotech, Biomaterials and Biomedical TechConnect Briefs 2017

Sensors - Chemical, Physical & Bio Chapter 7

Organic Nanobiomimetic Memristor/Memcapacitor Devices Ultrasensitive Direct Detect Matrix Matelloproteinase-2 in Human Serum

E.T. Chen, J. Thornton, S-H. Duh and P.T. Kissinger
Advanced Biomimetic Sensors, United States

pp. 271 - 274

Keywords: organic memristor/memcapacitor, nanobiomimetic crossing-nanotubes, inducing bio-communication, direct electron-relay transfer system, energy storage and sensing device, MMP2, probe-free and label-free

Matrix Matelloproteinase-2 (MMP-2) plays a key role in many diseases. A new type of dual-functioning device was developed for fast, direct ultrasensitive detection of MMP-2 using NIST SRM965A reference human serum specimens under label-free, probe-free and reagent-free conditions. The organic memcapacitor/memristor device was based on a self-assembled polymer membrane having polarized 3D array crossing-nanotube structures on a gold substrate that enables selective inducing bio-communication with MMP2. The characteristics of memcapacitor/memristor were observed in the i-V curves of the hysteresis. The Chronoamperometry (CA) method has a Detection of Limits (DOL) value of 8.67x10-18g/mL in PBS solution related to current density between 1.47 µA/cm2 and 919.2 mA/cm2 over MMP2 concentration between 20 ag/mL to 100 ng/mL with a Relative Pooled Standard Deviation 1.4% (n=26). The Double Step Chronopotentiometry (DSCPO) method produced a similar impression value of 1.47% (n=18) over MMP2 concentration 40 ag/mL to 100 ng/mL over energy density between 185 - 0.47 µWHR/cm3. The sensor was able to direct detect MMP2 in pure NIST serum specimens in the concentrations of 81.15 ± 0.10 ag/mL in normal glycemia serum, 1.13 ± 0.0016 pg/mL in hypoglycemia serum and 1.4± 0.0001 pg/mL in hyperglycemia serum, respectively. Multiple factors impacting on the sensor’s energy storage capability were also studied.